Sealing Film Peeling During Incubation

Written By: Risa Toth, Business Development Manager, Excel Scientific

A subtle problem with big consequences

Sealing film peeling during incubation is a silent source of experimental failure. Whether the film lifts at one corner or begins peeling across the surface, the consequences include evaporation, contamination, and inconsistent assay performance.

This issue appears across workflows from PCR to ELISA to cell culture.

Why Seals Peel During Incubation

1. Temperature Stress
   Films not rated for elevated temperatures (37°C, 60°C, 95°C) soften and lose adhesion.
2. Condensation and Moisture
   Moisture trapped during sealing may reduce adhesion strength, especially around the edges.
3. Plate Surface Variability
   Plates with textured rims or manufacturing oils may resist adhesives.
4. Incorrect Storage or Handling
   Using films stored in humid or dusty conditions reduces performance.
5. Using the Wrong Type of Film
   Breathable films for non-breathable applications
   Foil for fluorescence
   Optical film for heated incubation

How to Prevent Seal Peeling

1. Choose films engineered for your temperature range
   PCR grade films for thermocycling
   Incubation films for ELISA
   Breathable films for cell culture
2. Clean the plate rim if necessary
   A quick wipe with a lint-free cloth improves adhesion.
3. Apply pressure across the entire film surface
   A roller or plate sealing paddle helps achieve consistent contact.
4. Avoid re-positioning the film
   Once applied, peeling and re-seating weakens the adhesive.
5. Store seals in controlled conditions
   Dry, room temperature environments preserve adhesive quality.

Where Excel Scientific Fits In

Excel designs sealing films with high-performance acrylic adhesives that maintain adhesion across challenging temperatures and humid conditions. Their films resist curling, bubbling, and corner lifting even during extended incubations.

Recommended Excel Films

• SealPlate™  ideal non-peeling film for incubation
• AeraSeal™ breathability without loss of adhesion
• AlumaSeal™ robust adhesion for high-temperature or long-term storage